NeuroStem

We support research activities with the following services:

• Establishment of induced pluripotent stem cells (iPSCs) from patient cells using modern non-integrating approaches (mRNA transfection, Sendai virus, episomes).
• Quality assessment and control of the human pluripotent stem cell clones, including to the ready-for-immediate-publication level (as a Lab Resource and beyond).
• Design and implementation of the advanced precise gene variant engineering approaches, in the patient and select reference iPSC lines.
• Conceptualisation, experimental design and generation of advanced genetically-encoded reporters in human pluripotent stem cells, ranging from gene expression to specific intracellular process sensors.
• Comprehensive characterisation of the genetic consequences of genome engineering in stem cells, future-proofing of the key stem cell lines.
• Generation of neurons and other relevant brain/CNS-resident cell types, including specification of desired cell types by an enforced gene expression.
• Scaling up of all projects using a stem cell-dedicated, flexible facility-localised robotic system.
• Stem cell research-related consulting: NeuroStem can provide expertise and guidance on stem cell model design and development to maximise the quality and impact, including top-tier publication readiness, of your stem cell-based research.

• NeuroStem is led by scientists with an international profile in employing the most modern stem cell engineering technologies, including the latest and novel and bespoke genetic manipulation and transgenic tools, with a particular focus on applications to neuroscience.
• Experience in using innovative approaches in all aspects of stem cell engineering, from reprogramming into iPSCs to precise genome engineering to advanced transgenesis enabling gene expression visualisation and manipulation, reporters of (intra)cellular processes enabling high-throughput screening applications.
• Automation-facilitated ability to scale up any type of stem cell project, from iPSC generation to editing product evaluation and screening applications.
• Emphasis on the highest quality of experimental design and robustness of quality control, evaluation and screening of the genome engineering products, ensuring long-term robustness and streamlined publication of the resources.