Making spinal cord organoids from patient-derived induced pluripotent stem cells
Motor neuron disease (MND) is a neurodegenerative disease characterised by the progressive loss of motor neurons. With the advancement of stem cell technology, it has now become possible to use induced pluripotent stem cells (iPSC) derived from patients with MND to make mini brain and spinal cord structures in the lab.
This project will involve the generation of organoids from patient iPSC harbouring relevant mutations known to cause MND.
Comparing with iPSC-derived organoids from healthy control patients, we can investigate differences by studying individual cell types within the organoids as well as whole networks.
Organoids can be analysed using common biochemical techniques assessing gene and protein expression and there’s also the potential to live-image organoids over time to monitor cellular function with time. Further, using advanced electrophysiological techniques like microarray electrodes, it is possible to monitor the electrical activity of the organoids as a surrogate for synaptic networking which is known to be impaired in MND.
Latest Florey news for MND
Major funding boost for Florey MND research
The Florey’s Motor Neurone Disease research endeavours have been taken to new heights with a $3.2 million grant awarded by The Stafford Fox Medical Research Foundation which will see the team pursue new drugs targets for MND patients.
Motor neurone disease milestone: Patient trial shows impressive clinical results
A new drug delays motor neurone disease progression and improves cognitive and clinical symptoms according to recently announced trial results
Motor neurone disease: The inside story
The inside story behind recent clinical trial news. A new compound is offering real hope, and not only for MND, but there is a long way to go before we can claim success.
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